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Fig. 4-S2
Pharmacological treatments did not affect calcium activities of CNS neurons in living Tg (elavl3:Gcamp6s) zebrafish embryos.
(A) The schematic illustrates three different brain regions in zebrafish embryos. (B) Representative 72 hpf Tg (elavl3:Gcamp6s) embryos (top) and time courses of calcium transients (bottom) from individual neurons in living embryos that had been treated with the indicated pharmacological reagents. (C–E) Quantification of event rate of calcium transients from neurons in living 72 hpf Tg (elavl3:Gcamp6s) embryos that had been treated with the indicated pharmacological reagents used in this study. 10–20 neurons were randomly selected in the indicated three brain regions from three embryos per condition. (F–H) Average amplitudes of calcium transients from neurons in living 72 hpf Tg (elavl3:Gcamp6s) embryos that had been treated with the indicated pharmacological reagents used in this study. 10–20 neurons were randomly selected in the indicated three brain regions from three embryos per condition.