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Fig. 3
Two days post-fertilisation Tg(mpeg-1:mCherry) zebrafish embryos were injected into their HBV with Mock, BAC, Mock/M. marinum (Mmar) or BAC/Mmar. 10 ng Bac5 was injected for both Bac5 treatment groups and approximately 190 CFU Mmar expressing GFP for both infection groups. Z-stack images of the HBV region were acquired at 3 hours post-injection (hpi) and 24 hpi. (A-H) Representative fluorescence overlay images of fish at 3 hpi (A-D) and 24 hpi (E-H) from a single experiment are shown. Scale bar 100 μm. (I-J) Macrophages in the HBV region were quantified from fluorescence images of zebrafish taken at 3 hpi (I) and 24 hpi (J) using Icy Spot Detector plugin. Data pooled from three independent experiments is shown. Sample size (n): 40, 36, 61, 53. Error bars represent S.E.M. One-way ANOVA with Bonferroni’s post-test. *p<0.05, ***p<0.001.