ZFIN Image: Demal et al., 2019, Fig. 6

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Figure Caption

Fig. 6

Ectopic valvular tissue growths occur in adult zebrafish overexpressing the bmpr1aa^p.R438H variant within endocardium. (A,B) Electron microscopic images of adult zebrafish hearts with (A) Tg(fli1a:Gal4FF)^ubs3; Tg(UAS:bmpr1aa^WT_IRES_EGFP)^md65; Tg(7xTCF-Xia.Sia:NLS-mCherry)^ia5 or (B) Tg(fli1a:Gal4FF)^ubs3; Tg(UAS:bmpr1aa^p.R438H_IRES_EGFP)^md60; Tg(7xTCF-Xia.Sia:NLS-mCherry)^ia5. M = myocardium. Scale bar = 100 µm. (A′,B′) Magnified view of the atrioventricular valve. L = valve leaflet. Scale bar = 20 µm. (A) Adult zebrafish hearts with endothelial overexpression of bmpr1aa^WT do not have any obvious morphological changes. (B′) 3 out of 8 analysed adult zebrafish hearts overexpressing bmpr1aa^p.R438H have ectopic valvular tissue growth at the atrioventricular valve (arrows). (C) Numbers of zebrafish hearts overexpressing either bmpr1aa^p.R438H or bmpr1aa^WT with ectopic valvular tissue growth. None out of 7 analysed zebrafish hearts overexpressing bmpr1aa^WT had ectopic valvular tissue growths (p = 0.200, fisher’s exact test).

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Phenotype details

Acknowledgments

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