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Fig. 6
Hypothyroidism is evident among duox mutants. At 5 dpf, homozygous mutant larvae lack staining for bound T4 in the thyroid follicles, based on wholemount fluorescent immunohistochemistry (D,E). This is in sharp contrast to the robust staining observed in WT and heterozygous siblings (A–C). The NADPH oxidase inhibitor DPI successfully phenocopies duoxmutations in WT larvae, resulting in an absence of T4 detection (F). Scale bar: 50 μm.
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