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Fig. 5
Interaction of YSL-Exosomes with Endothelium and Presence of YSL-Exosomes in ISF
(A) Time-lapse of Video S5A, showing the rolling and arrest of exosomes in the cardinal vein of 3 dpf zebrafish embryo expressing CD63-pHluorin in the YSL.
(B) Quantification of dwelling time of CD63-pHluorin exosomes at the vascular wall (mean ± SD, n = 20 events).
(C) EM image of the CVP of a 3 dpf zebrafish embryo expressing CD63-pHluorin in the YSL, labeled with gold particles directed to GFP (10 nm). Insert indicates area shown at higher magnification to the right. Arrow indicates internalized CD63-pHluorin exosomes in endo-lysosomal compartment of endothelial cell.
(D) Close up of the cardinal vein of 3 dpf Tg(kdrl:Hsa.HRAS-mCherry) zebrafish embryos expressing CD63-pHluorin in the YSL, control-treated (DMSO), or treated with bafilomcinA (BafA) to neutralize acidic compartments.
(E) CVP area of 3 dpf Tg(kdrl:Hsa.HRAS-mCherry) zebrafish embryos sham-injected or injected with CD63-pHluorin pDNA in the YSL.
(F) Mid-trunk segment of 3 dpf zebrafish embryos expressing CD63-pHluorin in the YSL and mCherry under the ubiquitous RPL5 promoter. At this exposure level in the red channel, mostly muscle cells are visible. YSL, yolk syncytial layer; CVP, caudal vein plexus; PCV, posterior cardinal vein.
Reprinted from Developmental Cell, 48(4), Verweij, F.J., Revenu, C., Arras, G., Dingli, F., Loew, D., Pegtel, M.D., Follain, G., Allio, G., Goetz, J.G., Zimmermann, P., Herbomel, P., Del Bene, F., Raposo, G., van Niel, G., Live Tracking of Inter-organ Communication by Endogenous Exosomes In Vivo, 573-589.e4, Copyright (2019) with permission from Elsevier. Full text @ Dev. Cell