Fig. 5
Ginger increases the level of nitric oxide (NO) in non-transgenic WT and plcg1−/− mutant embryos. (A) Effect of ginger on NO staining at 1 dpf WT and plcg1−/− embryos. (B,C) Effect of NO donor (SNAP) and 2 Nos inhibitors (L-NAME, L-NMMA) on 2 dpf WT (B) and plcg1−/− embryos (C). Red arrows show ginger effect in CVP region. The effect of the NO donor SNAP is shown for comparison. Non-transgenic WT and mutant embryos are used to see the NO fluorescent dye. Pericardial edema, a typical phenotype occurred in plcg1−/− mutants at 2 dpf (C). Pictures are the representative images of ≥30 embryos per condition. Scale bars = 250 μm.