ZFIN Image: Ferri-Lagneau et al., 2019, Fig. 1

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Figure Caption

Fig. 1

Ginger rescues the dorsal aorta (DA) and the formation of hematopoietic stem/progenitor cells (HSPCs) in plcg1^−/−embryos. (A) Real-time imaging of the vasculature in tg(fli1:GFP) embryos at 30 hpf. Red rectangle shows the location of ISV. (B) In situ hybridization of the DA marker ephrin-B2a (efnb2a) at 1dpf (32 hpf). Black arrow indicates the artery, red arrow shows absence of artery in mutant fish. (C) In situ hybridization of the HSPC marker myb at 1 (32 hpf) vs 2dpf (54hpf). Black arrow points to myb expression in hemogenic endothelium (1 dpf) and CHT region (2 dpf), red arrow indicates absence of expression in mutant fish. (D) In situ hybridization of notch3 (normally expressed in the DA at 1 dpf (32 hpf)) and bmp7a (shown here for the first time to be expressed in the DA from 1(32 hpf) to 2 dpf (48 hpf)). These marker expressions are lost in plcg1^−/− embryos and rescued following exposure to ginger. Red arrow points to DA. Scale bars = 250 μm.

Acknowledgments

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