Fig. 2

Fig. 2

Behavioral, functional and biochemical parameters in zebrafish treated with MitoPQ vs. Paraquat. Zebrafish were treated with the indicated concentrations of MitoPQ and paraquat, or solvent (control), from 3 dpf until measurements (at 5 or 6 dpf). (A, B) Sensorimotor reflexes and spontaneous movement at 5 dpf: (Ai) tail and (Aii) head reflexes; (Bi) representative trajectories; (Bii) distance; (Biii) initiations, and (Biv) movement speed; n = 27–78 larvae; *P < 0.05 vs. control. (C) Protein carbonyl groups measured by dot-blot at 5 dpf. n = 3 independent experiments, *P < 0.05 vs. control. (D) Left – energy charge values, calculated with the indicated formula, at 5 dpf (triangles) and 6 dpf (circles); Right – representative HPLC chromatogram with adenine nucleotides; n = 3–4 independent experiments. (E) Heart rate and atrioventricular coordination at 6 dpf; n = 16–19 larvae.