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Fig. 1 rh activin A is detectable in zebrafish muscle tissue at 1 hpi. Before injection injury, adult zebrafish were anesthetized, and scales were gently removed from the right, dorsal side, just anterior to the dorsal fin (A). Zebrafish were injected at the descaled site with the indicated treatment using a microcapillary needle (B). To first test this model, adult Tg(Bre:GFP) zebrafish were injected with rh activin A (E: 5 × , H: 20 × ) or DMSO (D: 5 × , G: 20 × ), collected at 1 hpi, and analyzed for rh activin A expression by IHC. Act A was clearly detected at the Act A-injected injury site, and not in control DMSO or primary controls (C: 5 × , F: 20 × ). (F–H) Enlarged views of areas indicated by boxes in (C–E), respectively. n = 2 zebrafish per treatment group. (C–E) 5 × scale bar is 200 μm. (F–H) 20 × scale bar is 50 μm. Act A, activin A; DMSO, dimethyl sulfoxide; hpi, hours postinjury; IHC, immunohistochemistry; rh, recombinant human.