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Fig. 1 Expression patterns of pr72 and verification of pr72 knockout. (a, b) Time course expression of pr72 in zebrafish embryos at 0, 6, 12, 24, 48, 72 hpf and in adult hearts, identified by RT-PCR (a) and qPCR (b). (c) Expression of pr72 at 24, 48 and 72 hpf, as shown by whole mount in situ hybridization. A lateral view, anterior to the left, the expression in the heart is shown by red arrows. (d) A ventral view of the in situ result of embryos at 72 hpf, anterior to the top, the expression in the heart is indicated by a black arrow. (e, f & g) The sequencing results of TALEN target sites (indicated by the red frame) in PCR products amplified from F2 zebrafish (M1 (f) and M2 (g)) and the wild type (Wt); the restriction enzyme ScaII recognition site was shown using a black box in Wt. (h) The Pr72 Western-blot results of Wt, M1 and M2.