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Fig. 1 Permanent Scar Formation after Ventricular Resection in the Pachón Cavefish (A and B) Adult surface (A) and Pachón cavefish (B). (C–F) AFOG staining of the ventricular apex after resection. AFOG staining is a technique that stains myocardium orange and collagen blue. Both populations form a collagen scar (C and D, arrowheads), which disappears in the surface fish around 64 dpa (E), but persists in the Pachón (F). (G) Time line showing the reducing wound size in the surface fish but persisting wound in the Pachón. n ≥ 3 per population per time point, two-way ANOVA with Sidak’s test. (H) No difference in fin regeneration between Pachón (n = 18) and surface fish (n = 16) at 14 days post resection (dpr). Unpaired t test. (I and J) Regenerating dorsal lobes of tail fins of surface fish (I) and Pachón (J) at 14 dpr. Dotted line indicates the regenerated part. (K) Wound size 64 dpa in surface fish (n = 10), Pachón (n = 7), Tinaja (n = 6), Chica (n = 8), and surface fish × Pachón F1 hybrids (n = 5). One-way ANOVA with Tukey’s test. Detailed numbers and statistics are reported in STAR Methods Results are presented as mean ± SEM. All scale bars, 100 μm.