ZFIN Image: Hirsch et al., 2018, Fig. S5

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Figure Caption

Fig. S5

Segmental analysis of eTw-6 enhancer function in zebrafish embryos at 72 hpf.

(A) The eTw-6 enhancer that drives GFP expression in the pectoral and branchial arch was divided into four overlapping segments (1–4). The UCSC genome browser (http://genome.ucsc.edu) conservation track shows that segment 4 which contains Hdac9 exon 18 is conserved between humans and fish. (B) A graph displaying the number of embryos with GFP expression in the pectoral fin, branchial arch, brain and specific neurons out of all live embryos at 72 hpf. (C) Zebrafish enhancer assay results for eTw-6 segments: segment 1 and segment 4 did not drive GFP expression, while segments 2 and segment 3 drove GFP expression in neurons near the eyes that project to the trunk.

Acknowledgments

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