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Fig. S3
Characterization of the sclerotome.
Wild-type embryos at 24 hpf were co-labeled with neural crest markers crestin (A, green), mitfa (B, green), or the muscle pioneer marker eng2 (C, green), with nkx3.1 (red). nkx3.1-expressing dorsal sclerotome (short arrows), ventral sclerotome (long arrows), and sclerotome derived notochord associated cells (arrowheads) do not express either crestin, mitfa, or eng2. The notochord (n) is indicated by brackets in lateral views and dotted lines in transverse views. n = 15 embryos per staining. Scale bars: 50 μm.
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