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Fig. S11
vangl2 mRNA overexpression in fn1a/1b morphant wild-type embryos. (A) Time-lapse confocal image of late gastrula cell expressing memGFP. fn1a/1b morpholino (MO) injected wild-type embryo co-injected with synthetic vangl2 mRNA. (B,C) Quantitation of the total numbers of membrane protrusions formed by wild-type embryos injected with fn1a/1b MO and vangl2 mRNA (n=5 cells, 4 embryos). The data from wild type (WT), vangl2 mRNA injected wild-type embryos, and fn1a/1b MO injected wildtype embryos are shown for comparison. (D) Quantitation of the total percentage of polarized large protrusions. Average values are shown ± standard deviation. **P<0.01, ***P<0.001, ****P<0.0001; P values are versus wild type; one-way ANOVA significance test followed by Tukey HSD post-hoc tests. Scale bar, 5 μm.