ZFIN Image: Olcese et al., 2017, Fig. S11

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Figure Caption

Fig. S11

Rescue of hi1392Tg mutant phenotypes by injection of zebrafish pih1d3

(a, b) Representative clutches of 72 hpf hi1392Tg mutant embryos injected at one-cell stage with 250 pg of zebrafish pih1d3 mRNA, showing rescue of mutant phenotypes (b) compared to non-injected siblings (a). (c) Quantification of common phenotypes in 48 hpf hi1392Tg mutant embryos (red columns) versus embryos injected with 250pg of zebrafish pih1d3 mRNA (green columns). There was complete or significant rescue of ventral body axis curvature, pronephric cysts and abnormal cardiac looping (images not shown). Columns represent data from 3 separate experiments with n=29-111 per experiment. Error bars show standard deviation, significance calculated using two way ANOVA, p value *<0.05, **<0.005, ***<0.001.

Acknowledgments

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