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Fig. S3
rraga-/- mutants have normal number of cells expressing olig2.
(A) Analysis of olig2 mRNA expression at 3 dpf by whole mount in situ hybridization. Number of olig2 positive cells is similar in rraga-/- mutants and wildtype siblings. (B) Dorsal view of 4 dpf Tg(claudinK:GFP) zebrafish larvae shows that rraga-/- mutants express claudink:GFP, but at reduced levels. Genotypes were determined by PCR after imaging
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Reprinted from Developmental Cell, 47, Meireles, A.M., Shen, K., Zoupi, L., Iyer, H., Bouchard, E.L., Williams, A., Talbot, W.S., The Lysosomal Transcription Factor TFEB Represses Myelination Downstream of the Rag-Ragulator Complex, 319-330.e5, Copyright (2018) with permission from Elsevier. Full text @ Dev. Cell