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Figure Caption
Fig. 1-S3
Zebrafish skull phenotypes in different mutant combinations.
Dorsal views of adult zebrafish skull bones stained with Alizarin Red. We observed coronal suture loss (asterisks) in tcf12-/-; twist1b-/- mutants of any twist1a genotype (D–F). Animals lacking both Twist1 homologs occasionally develop ectopic sutures (arrows) in the anterior frontal bone region (B,C, enlarged in F). We also occasionally observed gaps (arrowhead, (D) and ectopic sutures (arrows, enlarged in G) in the posterior region of the parietal bone. Scale bars, 1 mm.
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