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Fig. 4
Comparison of ovarian size, morphology and spawning frequency between pgrmc1−/− and wildtype. (A) Gonadosomatic index (GSI) of wildtype (wt) and pgrmc1−/−. (B) HE staining of a representative ovarian section from a pgrmc1−/− female showed well-formed different stages of oocytes with a significantly higher number of Stage I follicles compared to wildtype (wt). Scale bars: 500 μm. (C) A higher number of Stage I follicles but a lower number of late stage follicles were observed in pgrmc1−/− compared to wt. (D) Mutant females spawned with less frequency than wt. *p < 0.05; **p < 0.01; ***p < 0.001.
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