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Fig. 2
Mga interacts with Bs69. (A) Confocal images of Mga and HA-Bs69 proteins in 7 hpf embryos. Scale bar 10 μm. (B) Co-immunoprecipitation of Mga and HA-Bs69 in nuclear lysates from 8 hpf embryos. (C) Co-immunoprecipitation of Mga and HA-Bs69 in cytoplasmic lysates from 8 hpf embryos. (D) Co-immunoprecipitation of HA-Bs69 and FLAG-Mga in 293T cells. (E) Identification of a conserved PXLXP motif in human, mouse and zebrafish MGAs at the end of the C-terminal region. The PXLXP conserved motif were highlighted in red. (F) Schematic representation of the full-length and truncated Mga and Bs69 constructs as indicated. (G) FLAG-MgaΔ did not immunoprecipitate with HA-Bs69 in 293T cells. (H) FLAG-Mga did not immunoprecipitate with HA-Bs69Δ in 293T cells. (I) FLAG-Mga-Cter immunoprecipitated with HA-Bs69-Mynd in 293T cells. (J) Co-immunoprecipitation assay of the in vitro translated FLAG-Mga-Cter and HA-Bs69-Mynd.