|
Fig. 3
Inheritance of conserved lncRNA Cyrano in zebrafish. (A) FPKM values of Cyrano expression in RNA-seq datasets of zebrafish testis, sperm, oocyte, 2cell-stage shows that Cyrano is present as early as 2cell-stage in zebrafish embryo. (B) RT-PCR validation of Cyrano and unspliced Cyrano expression in zebrafish testis, oocyte and 2cell-stage. (C) qPCR validation of Cyrano and unspliced Cyrano expression in zebrafish developmental stages. (D) Expression of Cyrano in zebrafish developmental stages checked by In situ hybridization. Error bar represents standard error of mean from N = number of independent biological replicates. *P value ≤0.05.