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Fig. 4
Anti-CNGA2a immunoreactivity is not affected following KO of cnga2a and/or cnga2b. (A) Immunostaining and confocal imaging of 6-day-old larva NPO of the wild type zebrafish (n = 30/30), cnga2a (n = 20/20), cnga2b (n = 16/16), and double cnga2a/2b (n = 6/6) zebrafish mutant variants with anti-CNGA (magenta) and anti-OXT (gray scale) antibodies. (B) Anti-CNGA2a mAb immunostaining and confocal imaging of the hypothalamus and neurohypophysis of 6-day-old larva injected with different concentrations of cnga2a missense morpholino oligonucleotide (n = 10/10). Scale bars: 10 μm.
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