ZFIN Image: Formella et al., 2018, Fig. 4

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Figure Caption

Fig. 4

KR-mediated ROS production leads to MN degeneration. (A) A transgenic zebrafish (Tg[met:Gal4;UAS:EGFP];Tg[UAS:MLS-KR]) mosaically expressing EGFP (green; ai) and KR (red, aii) in individual neurons prior to light illumination and KR-activation (aiii). (B) Light illumination of fluorescent neurons leads to bleaching of KR fluorescence, evident 60 min post-illumination (bii), while EGFP fluorescence intensities remained unchanged (bi). (C) Live imaging of KR activated neurons for up to 10 h revealed a series of morphological changes including progressive anterograde degeneration (ci-ciii, arrowheads), soma shrinkage (arrows) and MN death (asterisks). Scale bar 25 µm.

Acknowledgments

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