Fig. S2
There appears to be a change in pressure within the notochord at the start of extrusion. Close-up of notochord after tail excision to show cell membranes are bowed towards anterior at the start and then become bowed towards the posterior. The yellow arrowhead tracks one membrane. Not all fish show bowing to the anterior, the prevalence of this phenotype varies by age, position on the anterior/posterior axis and strain used. We have found the AB wildtype strain at 72hpf is most reliable. Images captured every 30 seconds as in Supplementary Movie 1. Numbers indicate time in minutes. Scale bar = 100μm.