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Fig. S4
Cancer model using TEAZ shows evidence of one tumor of mixed origin (n=1). (a) Fluorescent and brightfield imaging demonstrated a large primary tumor at the site of electroporation at 12 weeks post-electroporation with patchy GFP expression. (b) Imaging the zebrafish dorsally illustrates the large raised tumor from the contour of the animal. (c) Pathology of the tumor by hematoxylin and eosin or immunochemistry against GFP revealed that part of the deep tumor was GFP-negative and did not resemble a melanoma, but instead appeared consistent with a sarcoma. Images are visualized at 4x and 40x where scale bars represent 1 mm and 50 ?m respectively. The blue boxes represent the area of sarcoma enlarged at 40x and the red boxes represent the area of melanoma enlarged at 40x.