ZFIN Image: Callahan et al., 2018, Fig. 4

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Figure Caption

Fig. 4

Histological comparison of the embryo injection melanoma model and TEAZ melanoma model. (A) The left images show a melanoma created by injection of an mitfa:BRAF^V600E-tdTomato (fusion) transgene into a tp53^?/? background (n=1). Right images show a TEAZ-based melanoma created by electroporation of miniCoopR:GFP plus ubb:Cas9 plus zfU6:sgRNA against Rb1 (n=1) (example shown is fish at 16 weeks also shown in Fig. 2A). (B) H&E staining of both tumors shows similar histology, although with increased melanin pigmentation in the TEAZ tumor (also shown in Fig. 3A). (C,D) Antibody staining against BRAF^V600E shows that both tumors are widely BRAF^V600E positive, which correlates with high levels of phospho-ERK staining. (E) Reflecting the neural crest origin of melanocytes, both tumors show strong nuclear expression of SOX10. Images are visualized at 4� and 40�. Scale bars: 500 ?m (4�) and 50 ?m (40�). Dashed line boxes indicate the area enlarged at 40�.

Acknowledgments

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