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Fig. 2
Wnt/β-Catenin signalling is required for patterning during larval regeneration. a Expression of the Wnt ligand, wnt10a, and the downstream target, tcf7, become evident by 18 hpe (arrowheads) (wnt10a/18 hpe 7/8, wnt10a/24 hpe 10/10, tcf7/18 hpe 9/10, tcf7/24 hpe 10/10, number of experiments = 2). b Larvae treated with 10 μM IWR-1 continuously after excision show no signs of regeneration at 96 hpe (10/10 number of experiments = 2). c Heterozygous hs:dkk1 fish and their siblings were heatshocked by placing in an incubator at 39 °C and sorted based by fluorescence present in the transgenic line. Nonfluorescent fish served as control fish. The GskXV treatments were done at 10 μM (hs:dkk1/dlx5a 10/10, hs:dkk1/raldh2 10/10, number of experiments = 2) (GskXV/dlx5a 10/10, GskXV/raldh2 10/10, number of experiments = 4). d Expression of fgf10a and pea3 are downregulated in hs:dkk1 larvae (treatment as in panel c) and upregulated in fish treated with 12.5 μM GskXV (hs:dkk110/12 control, 28/30 transgenic, number of experiments = 2) (GskXV control 12/12, treated 11/12 number of experiments = 2). e Expression of the Wnt/β-Catenin target gene axin2 is reduced in fish treated with 10 μM SU5402 (12/12, number of experiments = 3)