ZFIN Image: Romero et al., 2018, Fig. S6

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Figure Caption

Fig. S6

Hedgehog signalling does not play a crucial role in fin fold regeneration. (A) Treatment with 20μM cyclopamine is sufficient to block regeneration and has a milder affect on fin fold excision. Regrowth was measured from images in Fiji/ImageJ software by placing a rectangle parallel to the body that started at the end of the notochord and finished at the caudal end of the fin fold (n = 7 for each sample, number of experiments = 2). (B) Neither ptch1 nor ihhb are noticeably upregulated following fin fold excision. Arrowheads indicate low levels of ptch1 expression in unoperated and fin fold excision larvae (10/10 for each sample except for ihhb/tail excision 9/10, number of experiments = 2).

Acknowledgments

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