ZFIN Image: Romero et al., 2018, Fig. S5

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Figure Caption

Fig. S5

shha is expressed in the notochord bead and Hedgehog signalling is unaffected by manipulation of other developmental pathways. (A) shha is expressed in the notochord bead (arrowheads) (n = 3/7 for 0hpe and 11/12 for 3hpe, number of experiments = 2). (B) The activity of the Hedgehog pathway was monitored by in situ hybridisation for ptch1. Larvae were treated with GskXV at 12.5μM from 40-48hpa to activate the WNT/β-Catenin pathway. hs:dkk1b-GFP fish were heatshocked for three hours at 39°C at 28hpa and allowed to recover for 17 hours at 28.5°C to inhibit the Wnt/β-Catenin pathway. Larvae were treated with SU5402 at 15μM from 44-48hpf to inhibit the FGF pathway. Larvae were treated with 100 μM DEAB from 1-48hpa to block the RA pathway. Larvae were treated with RA at 2.5μM from 43.5-48hpf to activate the RA pathway (10/10 for all panels, number of experiments = 2).

Acknowledgments

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