Fig. 5

A subset of mitfa negative iridoblasts is resistant to PKA signaling. (A–D) Brightfield images of dorsal trunks of wildtype or nacre/mitfa mutant larvae at 2 dpf treated with 0.1% DMSO or 5 µM forskolin and processed for in situ hybridization using a pnp4a probe. A reduction in pnp4a is detected in wildtype (as previously observed) but not in nacre/mitfa larvae. Scale bar = 100 um and applies to all images. (E) Quantification of differentiated iridophores in 0.1% DMSO or 5 µM forskolin-treated nacre/mitfa larvae. We note a significant, yet attenuated reduction in iridophores with mitfa loss of function (22.2% reduction in iridophores or 77.8% of control). The reductions are significant via Student’s t-test (p < 0.05).