Fig. 1
Adult LCs are largely derived from the VDA region in zebrafish.
(A) A schematic diagram indicates the RBI, VDA and PBI regions and the time point (4 dpf and 3 months) when the heat-shocked fish are analyzed. (B) Quantification of the percentage of GFP+ LCs derived from the RBI, VDA, PBI, and non-heat-shocked control at 4 dpf and adulthood. n = 13, 7, 10 and 6 for the RBI-, VDA-, PBI-, and non-heat-shocked control fish analyzed at 4 dpf respectively. n = 5, 5, 5 and 6 for the RBI-, VDA-, PBI-, and non-heat-shocked control fish analyzed at adulthood, respectively. Error bars represent mean SEM. ***p<0.001. (C) Anti-GFP staining shows that GFP+ LCs are detected in the RBI-labelled fish, but not VDA- and PBI-labelled fish at 4 dpf. The red box indicates the imaging region. (D) Anti-GFP staining indicates that GFP+ LCs are mainly detected in the VDA-labelled fish, but not the RBI- and PBI-labelled fish in adulthood. The red box indicates the imaging region.