|
Fig. S4
A chemical inhibitor of Bmp signalling restores the area of the atrial cardiac jelly back to levels of siblings. A. Confocal stacks showing the hearts of sibling and double mutant embryos at 72 hpf in the Tg(ubi:ssNcan-EGFP) background treated with either DMSO control or the Bmp signalling-specific inhibitor, DMH1. Embryos were incubated for 24 hours prior to live imaging and the area of the cardiac jelly quantified for each treatment. B. Dot plots of cardiac jelly area for individual embryos following treatment with either DMSO or DMH1. Double mutant embryos had significantly increased cardiac jelly area compared with sibling controls; however this increase was restored to the levels of siblings, following Bmp inhibition with DMH1. DMSO siblings n=14, DMSO double mutants n=13, DMH1 siblings n=12, DMH1 double mutants n=11. Statistics was determined using a two-way ANOVA for multiple comparisons with Tukey’s post hoc correction. *p < 0.05. ns=not significant. Error bars depict std dev.