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Fig. S10
Apelin receptor signaling is not essential for ectopic endoderm ingression.
(A-B) Schematic diagrams depicting single donor transplant assay to test the role of apelin receptor signaling. (A) acvr1ba*-expressing cells with aplnra and aplnrb MOs were transplanted to the animal pole of a wild-type host embryo. (B) Cells with aplnra and aplnrb MOs alone were transplanted to the animal pole of a wild-type host embryo. (C-D) Representative images showing distribution of induced endodermal cells in a wild-type host. Donor cells in (A) (green) mainly localized to endoderm-derived tissue (C), while donor cells in (B) mainly localized to ectoderm-derived tissue (D). Lateral view, anterior to the right. (E) Boxplot quantification of endoderm contribution at 21 hpf of transplanted cells depicted in (AB). acvr1ba*-expressing cells with aplnra and aplnrb MOs contributed to endoderm significantly more than cells with aplnra and aplnrb MOs alone. Data is shown as mean ± SEM of 3 independent transplantation experiments with 18 embryos per condition. Student’s t-test was performed. *** p<0.001.