Fig. 1
Setup of an ex vivo system for an isolated zebrafish heart. (A) Equipment needed, including a dissecting microscope (1), with a camera (2), pump (3) and stimulator (4). (B) Two-line 34G catheters (5) are placed on a Sylgard-coated Petri dish with the use of insect pins; 2 hearts are tied on the ends of the catheters (inset depicts the position of a cannula in the heart; arrowhead points to the place of the tie by the atrioventricular junction), which are put in front of a mirror (6); platinum electrodes (7) are used for electrical field stimulation. A pool (8) was made to control the solution level. (C) Diagram of the perfusion system.