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Fig. S2
Axon pathfinding defects of ATG1 and ATG2 morphants are not associated with obvious alterations of muscle fibres. (A) Immunolabelling of fast (F310 antibody) and/or slow (F59 antibody) muscle fibres in 26-, 56- or 72-hpf embryos injected with MOscr, MOspATG1 or MOspATG2 morpholinos. Slow and fast muscle fibres are well specified and develop properly in morphant embryos or larvae compared to controls. ATG2 morphants only show a slight developmental delay. (B) Co-immunolabelling of sMN axons (zn-5 antibody) and muscle fibres (phalloïdin) in 72-hpf control, ATG1 and ATG2 morphant larvae. Dotted lines indicate the horizontal myoseptum. Arrows point at control rostral nerves, while full arrowheads and empty arrowheads respectively indicate misguided or missing rostral nerves in ATG1 or ATG2 morphants. Secondary motor axons of ATG1 and ATG2 morphant larvae fail to navigate properly in an apparently normal muscular environment. (A-B) Scale bars: 50 μm.