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Fig. 7
Mypt1-202 expression can reduce Mypt1 loss-of-function in zebrafish embryos. Lateral views of representative 48 hpf zebrafish embryos injected with (A) control morpholino, (B) 1.0 ng ppp1r12a MO, (C) a partially rescued embryo injected with 25 pg ppp1r12a-tv202 mRNA and 1.0 ng of ppp1r12a MO. (D–F) Embryos at bud stage stained with hgg1 (to mark the prechordal plate), shh (midline), pax2.1 (midbrain-hindbrain boundary) and dlx3 (neural plate) after injection of (D) control morpholino, (E) ppp1r12a MO or (F) ppp1r12a-tv202 mRNA and ppp1r12a MO. (G) Quantification of the truncated body axis phenotype in morphant and mRNA injected embryos. Error bars are standard error and a* indicates a statistically significant (p < 0.05) difference from control and a# indicates a statistically significant (p < 0.05) rescue compared to the corresponding morpholino injected embryos. Injections were performed a minimum of three times with 50 total embryos measured. Embryos were collected by spawning heterozygotic hi2653 adult zebrafish (I and J) or from WT controls (H). Mutant embryos were injected with 25 pg of ppp1r12a-tv202 mRNA at the one cell stage (I). Embryos were collected at 36 hpf and probed with prox1 to mark the liver primordium (Marked with an arrowhead). Liver staining for prox1 was detected in 68 of 68 wild-type embryos, 57 of 77 uninjected embryos from the heterozygotic cross and 89/91 rescued embryos.
Reprinted from Gene, 675, LaFlamme, A., Young, K.E., Lang, I., Weiser, D.C., Alternative splicing of (ppp1r12a/mypt1) in zebrafish produces a novel myosin phosphatase targeting subunit, 15-26, Copyright (2018) with permission from Elsevier. Full text @ Gene