ZFIN Image: Tabor et al., 2018, Fig. 6

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Figure Caption

Fig. 6

Presynaptic Inhibition Mediates Long-Interstimulus Interval Prepulse Inhibition

(A) Startle responsiveness (% SLC) to an acoustic pulse (black) or an electric field pulse (red) at intervals of 100 to 1,000 ms after an acoustic prepulse. n = 9 groups of 20 larvae. Error bars are SEM. ^∗Paired t test, p < 0.05.

(B) Projection showing the auditory ganglia (y256-Gal4, red) and Mauthner cells (y264-Gal4, black). Right: auditory nerve expressing iGluSnFR (y256-Gal4, UAS:iGluSnFR, red) and the Mauthner lateral dendrite (black).

(C) Mean iGLuSnFR signals of VIII^th nerve clusters (color-coded) in response to pulse alone (left traces) and PPI trials (right traces). Arrowheads: time of a prepulse (small) and pulse (large). Black outline: Mauthner lateral dendrite. Scale bar, 50 μm.

(D) PPI of iGluSnFR signal in VIII^th nerve segments adjacent to the Mauthner dendrite (Apposed), or not (Distant). n = 10 larvae. ^∗WSR test, p = 0.002. Red in (D)–(F) is mean ± SEM.

(E) iGluSnFR PPI for apposed segments at 100 and 500 ms interstimulus interval (ISI) PPI tests. n = 4 larvae. ^∗WSR test, p = 0.02.

(F) iGluSnFR PPI for apposed segments when Mauthner action potential was suppressed (No spike) or present (Mc spike). n = 9 larvae. ^∗WSR test, p = 0.04.