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Fig. 6
Presynaptic Inhibition Mediates Long-Interstimulus Interval Prepulse Inhibition
(A) Startle responsiveness (% SLC) to an acoustic pulse (black) or an electric field pulse (red) at intervals of 100 to 1,000 ms after an acoustic prepulse. n = 9 groups of 20 larvae. Error bars are SEM. ?Paired t test, p < 0.05.
(B) Projection showing the auditory ganglia (y256-Gal4, red) and Mauthner cells (y264-Gal4, black). Right: auditory nerve expressing iGluSnFR (y256-Gal4, UAS:iGluSnFR, red) and the Mauthner lateral dendrite (black).
(C) Mean iGLuSnFR signals of VIIIth nerve clusters (color-coded) in response to pulse alone (left traces) and PPI trials (right traces). Arrowheads: time of a prepulse (small) and pulse (large). Black outline: Mauthner lateral dendrite. Scale bar, 50 ?m.
(D) PPI of iGluSnFR signal in VIIIth nerve segments adjacent to the Mauthner dendrite (Apposed), or not (Distant). n = 10 larvae. ?WSR test, p = 0.002. Red in (D)?(F) is mean ± SEM.
(E) iGluSnFR PPI for apposed segments at 100 and 500 ms interstimulus interval (ISI) PPI tests. n = 4 larvae. ?WSR test, p = 0.02.
(F) iGluSnFR PPI for apposed segments when Mauthner action potential was suppressed (No spike) or present (Mc spike). n = 9 larvae. ?WSR test, p = 0.04.
See also Figure S6.