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Fig. S9
Analysis of mutant dvl2 and dvl3a transcripts.
(A) Semi-quantitative RT-PCR analysis to detect mutant dvl2 and dvl3a transcripts at 1-cell stage. ß-actin served as a loading control. NMD can be observed for dvl2 and dvl3a transcripts, respectively. (B, C) Quantification of mutant dvl2 and dvl3a mRNA levels in Mdvl2 and MZdvl3a mutants. The expression level in WT embryo is set as 1 after normalization with ß-actin. Bars represent the mean ± s.d. from three experiments (*, P<0.05; ****, P<0.0001). (D, E) In situ hybridization analysis of dvl2 transcripts in WT and Mdvl2 embryos. RT, reverse transcriptase.
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