ZFIN Image: Cantaut-Belarif et al., 2018, Fig. S4

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Figure Caption

Fig. S4

The sub-commissural organ is immunoreactive for the Reissner fiber material in mutants with defective cilia, related to Figure 4.

Z projection of stacks of dorsal optical sections (depth = 23 - 26 μm) of 48 hpf embryos immunostained against Acetylated-tubulin (magenta) allowing to detect axonal tracts (POC: post-optic commissure, PC: posterior commissure, arrowheads), and the Reissner fiber material (green) in the sub-commissural organ (SCO, double arrowheads). Control embryos (here an iguana sibling) as well as iguana, oval, elipsa and kurly mutants show immunoreactivity for the Reissner fiber material in the SCO. Rostral, top. e: eye. Scale bar represents 50 μm.

Acknowledgments

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