Fig. S1
Tg[mnr2b-hs:Gal4] is the stronger Gal4 driver for abducens motor neurons than Tg[mnGFF7]. (Related to Figure 1)
(A, B) Structures of Tg[mnr2b-hs:Gal4] and Tg[mnGFF7] (Asakawa et al., 2013) in the mnr2b BAC. (C-H) Lateral views of the whole bodies (C, D) and dorsal views of the caudal brains (E, F) at 5 dpf. Double arrowheads indicate the abducens nerve terminals. (G, H) Dorsal views of the caudal hindbrains and rostral spinal cords at 46 hpf. A transgenic line driving EGFP by mnr2b enhancers (mnr2b-GFP) (Asakawa et al., 2012) was used as a reference. sm, spinal motor neuron. The white and black arrows indicate the ventral clusters of the abducens nuclei in r5 and r6, respectively, in E-H. The rostral is to the top in E-H. The bars indicate 200 base pairs (A and B), 250μm (C and D) and 50 μm (F and G). Panels C and E are the same as in Figures 1B and 1C, respectively.