ZFIN Image: Ashlin et al., 2018, Fig. S4

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Figure Caption

Fig. S4

IGFBP2 dampens IGF signaling in zebrafish larvae, related to Figure 4.

(A-C’) Confocal images of 48 hpf tailfins labeled with anti-pIGFR antibody (green) reveals numerous puncta in both pitpnc1a ^-/- (B, B’) and pitpnc1a ^+/+ (C, C’) embryos, which are nearly eliminated after soaking in 1 ng/μL IGFBP2 (C, C’). D) IGFBP2 dose-dependently reduces brain c-fos levels and reduces growth in pitpnc1a ^-/- embryos. E-G) pitpnc1a ^-/- animals have slightly larger brains than pitpnc1a^+/+ larvae. E) Inter-ocular distance (red line) and brain length (green) were measured relative to total body length (not shown). F) Mutant brains are significantly (4.2%) larger than wild type (p=0.03, one-tailed t-test) as measured by interocular distance G) and trending (1.3%) larger by length (p=0.13, one-tailed t-test). Scale bars, A-C’, 20 μm.

Acknowledgments

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