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Fig. S2
Knock down of Cntn2 expression with antisense MO
Top panels show dorsal view of hindbrain, with anterior to the left, in embryos labeled with the anti-Cntn2 antibody. (A, B) Cntn2 is highly expressed in a control MO-injected embryo (A). Cntn2 expression is almost completely eliminated in a cntn2 MO-injected embryo (B). (C) Western blot analysis shows severe reduction of Cntn2 protein in cntn2 MO-injected embryos at 48 hpf. Scale bar in B, 50 μm for A and B.
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Reprinted from Mechanisms of Development, 152, Gurung, S., Asante, E., Hummel, D., Williams, A., Feldman-Schultz, O., Halloran, M.C., Sittaramane, V., Chandrasekhar, A., Distinct roles for the cell adhesion molecule Contactin2 in the development and function of neural circuits in zebrafish, 1-12, Copyright (2018) with permission from Elsevier. Full text @ Mech. Dev.