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Fig. 3-S1
Eda signaling during zebrafish scale development.
(A) Eda mutant (nkt/nkt) larvae lacked scales and had profound fin growth defects (n = 6). (B) eda is downregulated in epidermis overlying nascent scale papillae (b’ pre scale, arrow). Subsequently, eda is strongly expressed in dermal scale forming cells where it persists throughout scale development (B’’ papilla; B’’’ extension). n = 6. (C) edar expression was complementary to eda. In (C’ pre scale) skin, edar expression appears in epidermis in the region where eda is downregulated. edar expression remains epidermal (c’’ papilla) before shifting to the epidermal posterior margin (C’’’ extension). n = 6. Scale bars, 1 mm (A); 100 µm (B–D); 50 µm (E,F).