ZFIN Image: Oosterhof et al., 2018, Fig. 4

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Figure Caption

Fig. 4

RNA Sequencing Reveals Increased Expression of Genes Associated with Chemotaxis in csf1ra^−/−;b^+/− Mutant Microglia

(A) Schematic representation of the RNA sequencing experiment. Microglia were sorted by FACS from dissected brains from WT (3 brains per sample, 2 samples), csf1ra^−/− (3 brains per sample, 2 samples), and csf1ra^−/−;b^+/− (4–5 brains per sample, 2 samples) zebrafish.

(B) Multidimensional scaling plot.

(C) Heatmap of differentially expressed genes between csf1ra^−/−;b^+/− and WT microglia.

(D) Volcano plot of differentially expressed genes (csf1ra^−/−;b^+/− versus WT) whose expression values in csf1ra^−/− mutants lay between those of WT and csf1ra^−/−;b^+/− mutants.

(E and F) Gene ontology analysis was performed on genes that showed a csf1r-dependent decrease in expression (E) and increase in expression (F).

(G) Heatmap with expression Z scores for all chemokines and chemokine receptors that are expressed in zebrafish microglia.

Genes were differentially expressed with FDR < 0.01 and LogFC > |2|. Adult zebrafish used were between 9–12 months old.

Acknowledgments

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