Fig. S3
Genetic lineage tracing of tbx1+ cells, Related to Figure 4.
(A-D) Merged brightfield images and confocal z-stacks of 72 hours post-fertilization (hpf) Tg(tbx1:mKateP2Acre), Tg(ubi:HULK) double transgenic embryos (n=40) immunostained to detect GFP. Higher magnification views of (A) are shown in (B), (C), and (D). White arrows highlight labeled cells in the parietal pericardium (pp), cranial vasculature (cv), superior rectus (sr) ocular muscle, lateral rectus (lr) ocular muscle, and ocular vasculature (ov). Fluorescence in the lens (*) results from a cry:GFP selectable marker on the Tg(ubi:HULK) vector backbone. Lateral views, anterior left. Little to no variation was observed between animals in each experimental group. Abbr: v, ventricle. Scale bars=100 μm.