Fig. 2
Effects of tanshinol on bone mineralisation of wild-type AB strains zebrafish larval skull. Images of (A) the dorsal aspect head bone stained with alizarin red in wild-type AB strains zebrafish larvae at 9 days' postfertilisation (dpf) with or without exposure to tanshinol (0.5?M, 1?M, 2.5?M, 5?M); (B) the effect of tanshinol on bone mineralisation area in 9-dpf zebrafish; and (C) the effect of tanshinol on bone mineralisation integral optical density in 9-dpf zebrafish. Con = control, egg water; IOD = integral optical density; T0.5 = tanshinol 0.5?M; T1 = tanshinol 1?M; T2.5 = tanshinol 2.5?M; T5 = tanshinol 5?M. Data are given as mean ± standard deviation (n ? 9). *p < 0.05 versus control.