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Fig. 2
The effects of triptolide on podocin expression and distribution in MTZ-treated Tg(pod:gal4;UAS:NTR-mCherry) zebrafish. (A) The expression and distribution of podocin in different groups was measured and examined at 24?h, 30?h and 36?h after MTZ treatment in 84 hpf larvae; (a1?a3) normal control (CTL); (b1?b3) MTZ model group; (c1?c3) MTZ?+?triptolide group. (B) No obvious mean immunofluorescence intensity (GL) differences were observed between the three groups at 30?h and 36?h. (C) Positively stained area to the glomerular area (AR) revealed a remarkably reduction at 36?h in the MTZ model group, but remained strong in the triptolide treatment group. (n?=?3), **P?<?0.01, (original magnification, x1000).