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Fig. 7
Expression of nicotinic acetylcholine receptors (nAChRs) in 2 dpf embryos of controls, 6?mg/L THC and 3?mg/L CBD-treated fish. (A?D) ?-bungarotoxin labelling of postsynaptic membranes at the NMJ in zebrafish trunk musculature. (E) Bar graph representing the total number of ?-bungarotoxin puncta counted over per 1000 ?m2 area and compared between vehicle control (n?=?10) and 6?mg/L THC-treated embryos (n?=?6). (F) Bar graph representing the number of ?-bungarotoxin puncta with a minimum area of ~5 ?m2, compared between vehicle control (n?=?10) and 6?mg/L THC-treated embryos (n?=?6). (G) Bar graph representing the total number of ?-bungarotoxin puncta counted over a 1000 ?m2 area and compared between vehicle control (n?=?8) and 3?mg/L CBD-treated embryos (n?=?7). (H) Bar graph representing the number of ?-bungarotoxin puncta with a minimum area of ~5 ?m2, compared between vehicle control (n?=?8) and 3?mg/L CBD-treated embryos (n?=?7). * Significantly different from vehicle controls, p?<?0.05. **Significantly different from vehicle controls, p?<?0.01.