Fig. S10

Fig. S10

p47^phox morpholino deceases superoxide production in activated neutrophils.

Tg(mpx:GFP) embryos were injected at the one cell stage with either p47^phox morpholino (MO p47^phox) or a control morpholino (MO CTRL). Morphants were infected in the muscle with GFP- E. coli and superoxide was detected with DHE at 3 hpi. (A) Larvae images are representative fluorescence images of DHE at 3 hpi. Scale bar: 100 μm. White boxes indicate the position of the confocal images in (B). (B) Representative overlay of GFP fluorescence (neutrophils+E. coli) with DHE fluorescence (maximal projections of confocal images) show superoxide in neutrophils in control morphants but not in p47^phox morphants. Scale bars: 50 μm. (C-D) Dot plots are quantification of recruited DHE⁺ cells (C), recruited neutrophils (D), and recruited DHE⁺ MPX⁺ cells (E) in CTRL and p47^phox morphants (mean number of cell/larva ± SEM, N_{MO CTRL} = 13, N_{MO P47} = 13, Mann-Whitney test, two-tailed, ***p<0.001, ns: p>0.05 non significant). Diagrams show the counting region.