ZFIN Image: Phan et al., 2018, Fig. S9

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Figure Caption

Fig. S9

NADPH oxidase inhibitor VAS2870 increases the susceptibility of larvae to notochord infection.

(A) Experimental scheme. VAS2870 or DMSO was injected in the yolk of tg(lyz:DsRed) or tg(mpx:GFP) embryos at 2 dpf. One hour later, fluorescent E. coli bacteria were injected in the notochord and the injected embryos were scored from 1 dpi. (B) Representative fluorescent images of neutrophils in the VAS2870 or DMSO treated tg(mpx:GFP) embryos at 1 day post treatment (dpT) without bacterial injections. Scale bar: 400 μm. (C) Counts of total neutrophil population in indicated conditions at 6 hours (hpT) and 1 dpT (mean ± SEM, N_DMSO = 29–30 and N_VAS2870 = 25–29, Mann-Whitney test, two-tailed, p>0.05, ns = not significant). (D) Trunk images are representative overlays of DsRed (neutrophils), GFP (E. coli) and transmitted light images at 1 dpi in PBS- or E. coli- injected larvae in DMSO or VAS2870 treatment conditions. Scale bars: 100 μm. White arrowheads: E. coli in the notochord. (E) Survival curves of larvae injected with either PBS or E. coli from 0 to 3 dpi in DMSO or VAS2870 treatments (N_larvae is indicated in the figure, log rank test, ***p<0.001, from three independent experiments). (F) Larva phenotypes and bacterial outcome were scored from 0 to 3 dpi. (w/o: without bacterial growth, the number of larvae (N) is indicated each the column, from three independent experiments).

Acknowledgments

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