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Fig. S4
zebrafish hhex mutants exhibit increased hhex expression and the hhexs722 allele appears to encode a non-functional protein (a-b) Whole-mount in situ hybridization showing hhex expression in 32 hpf hhex+/+ and hhex-/- embryos. At 32 hpf, hhex-/- exhibit increased hhex expression in the vasculature (arrowheads point to ISVs). (c) hhex and hhexs722 mRNA injection at 50, 100 and 200 pg into one-cell stage wild-type embryos. Injection of wild-type hhex mRNA, but not hhexs722 mRNA, leads to developmental defects. Scale bars: 100 μm.
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Full text @ Nat. Commun.